Please note all intermediary steps you used to reach the final results (with details for all non-default parameters).

You would like to derive primers found in regions common to all 3 mRNA sequences aligned using the protein sequences as guides.

a) do a multiple sequence alignment (MSA) of the 3 proteins (emma)

b) align the DNA sequences over the protein MSA (tranalign).

c) derive a consensus for perfectly conserved regions (cons).

d) extract 5 potential primer pairs (eprimer3)

Give the 5 pairs and the positions of the amplified fragment (print eprimer3 results).

What is the size of the largest amplified fragment?