Optimizing the design of a microarray experiment
Friday August 25, 2006
| Microarray experiments are large-scale experiments used to measure the mRNA level of thousands of genes simultaneously. These experiments are quite expensive in terms of equipment, consumables and time, therefore a careful design is particularly important to maximize the information extracted and minimize the costs. We first present a short overview of the different key steps in a microarray experiment. We then focus on experimental design issues arising by gene expression on two-color spotted cDNA microarrays, but many of the same issues apply to single-color gene expression arrays as well. Experimental plans should ensure that the main effects are not confounded with secondary undesirable effects. Some real case studies will be shown. Participants are also encouraged to submit their questions in advance and eventually propose a design that will be discussed and commented during the course. |
| Teachers: M. Delorenzi (ISREC-NCCR, SIB) E. Migliavacca (ISREC-NCCR, SIB) |
| Location: reading room of the Genopode Building (Quartier Sorge, 1015 Dorigny) |
| Time | Topic | Speakers |
|---|---|---|
| 08h30 -12h00 | Optimizing the design of a microarray experiment | M. Delorenzi E. Migliavacca |
| 12:00 | Lunch | |
| 13h00 - 16h00 | Practicals Exam |
Staff |
| Supplementary material: |
|---|
Starting with "R" (word document) R objects & affymetrix annotation file used in the practicals: (right-click to download) ApoAI.RData.zip (2.8 MB) estro.RData.zip (8.1 MB) HG_U95Av2_annot_short.csv.zip |
Registration is closed
Participants:
(16 submissions)| Status | Firstname | Lastname | Departement | |
|---|---|---|---|---|
PhD student |
Monica |
Albarca Aguilera |
CIG |
|
PhD student |
Nicole |
Casson |
IMUL |
|
PhD student |
Alexandre |
Colard |
DEE |
|
PhD student |
Mara |
Colzani |
CIG |
|
PhD student |
Anne-sophie |
De Preux |
DGM |
|
Other |
Christian |
Fankhauser |
CIG |
|
Other |
Otto |
Hagenbüchle |
DAFL |
|
PhD student |
Matthew |
Hall |
CIG |
|
Group leader |
Liliane |
Michalik |
CIG |
|
Post-doc |
Michael |
Nicolas |
DEE |
|
PhD student |
Mingkwan |
Nipitwattanaphon |
DEE |
|
Technician |
Alexandra |
Paillusson |
CIG |
|
PhD student |
Genta |
Plasari |
LBTM |
|
PhD student |
Jaime |
Reina |
CIG |
|
Post-doc |
Zofia |
Terreau-Haftek |
CIG |
|
PhD student |
Yannick |
Wurm |
DEE |
Latest update 2006-08-25
